CyFlow™ CD1c PE
| Antigen: | CD1c |
| Alternative Name: | CD1, R7 |
| Clone: | L161 |
| Application: | Flow cytometry |
| Format/Fluorochrome: | PE |
| Laser: | Blue, Green, Yellow |
| Target Species: | Human |
| Field of Interest: | Immunophenotyping |
| Species of Origin: | Mouse |
| Regulatory Status: | RUO |
| Clonality: | monoclonal |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Isotype: | IgG1 |
| Quantity | 100 tests |
| Volume | 1 |
| Immunogen | Human thymocytes |
| Background Information | CD1c (R7, BDCA1) together with CD1a and CD1b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with α/β and γ/δ T cells. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Todate A, Chida K, Suda T, Imokawa S, Sato J, Ide K, Tsuchiya T, Inui N, Nakamura Y, Asada K, Hayakawa H, Nakamura H: Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis. Am·J·Respir·Crit·Care·Med. 2000·Jul; 162(1):148‑53. <·PMID:·10903234·> | Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA: Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J·Exp·Med. 2000·Jul·17; 192(2):281‑8. <·PMID:·10899914·> | del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L: Membrane trafficking of CD1c on activated T cells. J·Leukoc·Biol. 2001·Oct; 70(4):567‑77. <·PMID:·11590193·> | Gutzeit C, Raftery MJ, Peiser M, Tischer KB, Ulrich M, Eberhardt M, Stockfleth E, Giese T, Sauerbrei A, Morita CT, Schönrich G: Identification of an important immunological difference between virulent varicella‑zoster virus and its avirulent vaccine: viral disruption of dendritic cellinstruction. J·Immunol. 2010·Jul·1; 185(1):488‑97. <·PMID:·20525895·> | Scharf L, Li NS, Hawk AJ, Garzón D, Zhang T, Fox LM, Kazen AR, Shah S, Haddadian EJ, Gumperz JE, Saghatelian A, Faraldo-Gómez JD, Meredith SC, Piccirilli JA, Adams EJ: The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation. Immunity. 2010·Dec·14; 33(6):853‑62. <·PMID:·21167756·>
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