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CyFlow™ CD3 PE-Cy7

1.825,25 DKK*

Prices net excl. VAT, plus applicable shipping costs

Antigen:	CD3
Alternative Name:	Leu4, T3
Clone:	SK7
Application:	Flow cytometry
Format/Fluorochrome:	PE-Cy7
Laser:	Blue, Green, Yellow
Target Species:	Human, Non-Human Primates
Field of Interest:	Immunophenotyping
Species of Origin:	Mouse
Regulatory Status:	RUO
Clonality:	monoclonal
Emission Maximum:	785 nm
Excitation Maximum:	496 nm, 565 nm
Isotype:	IgG1

HLDA Workshop	HLDA II—WS Code T118 HLDA III—WS Code T492
Quantity	100 tests
Volume	0.4
Immunogen	Human thymocytes
Background Information	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Usage	The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage Buffer	The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Specific References

| Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non‑T cells in the acquired immunodeficiency syndrome. Am·J·Pathol. 1985·Sep; 120(3):371‑9. <·PMID:·3898858·> | Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti‑human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005·Dec; 116(4):487‑98. <·PMID:·16313362·> | Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Høybjør S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J·Immunol. 2007·Feb·15; 178(4):2556‑64. <·PMID:·17277165·> | Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T‑cell hyporesponsiveness. J·Invest·Dermatol. 2008·May; 128(5):1182‑91. <·PMID:·18239614·>

Available Technical Data Sheets

CyFlow™ CD3 PE-Cy7 (SK7) BQ006366 TDS (US)